You need to make 7.5 mL of a 10% yeast solution in Na-K-PO4 buffer. How many mL of buffer will you need?
Solution preparation,
To make 7.5 ml of 10% yeast solution
volume of yeast solution = 10 x 7.5/100
= 0.75 ml
therefore,
ml of buffer needed = 7.5 - 0.75
= 6.75 ml
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