Question

Select the BEST answer in regards to dissolving your sample and the developing solvent for TLC....

Select the BEST answer in regards to dissolving your sample and the developing solvent for TLC.

1.

The Rf value of your sample will be different relative to how long you run your TLC plate

2.

The Rf value of your sample will be different depending on the developing solvent used

3.

The developing solvent MUST be the same as the solvent used to dissolve your sample

4.

The Rf value of your sample will be different depending on the solvent you dissolve it into

5.

The developing solvent and solvent used to dissolve your sample do not need to be volatile

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Answer #1

2. The Rf value of the sample will be different depending on the developing solvent used. Polarity of the solvent used affects the Rf value of the sample. Solvent with too low or too high polarity should be avoided. If you use a solvent with too low polarity, the sample wont move at all, and using very high polarity solvent may lead to all of the compounds running right up the paper, leading in poor separation.

Thus, the Rf value depends on the developing solvent used.

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