Question

Biochem question

The enzymes D-amino acid oxidase has a very high turnover number because the D-amino acids are potentially toxic. The K_M for the enzyme is in the range of 1 to 2mM for such aromatic acids and in the range of 15 to 20 mM for such amino acids as serine, alanine, and the acidic amino acids. Which of these amino acids are the preferred substrates for the enzyme?

Answer 1

1) K_m is dissociation constant for the ES complex

2) K_m is a measure of how tightly the substrate is bound to the enzyme.

3) The greater the value of K_m the less tightly the substrate is bound to the enzyme.

4)  K_m often used to estimate the affinity of the enzyme for the substrate.

As K_m for aromatic D-amino acids is 1 to 2 mM and 15 to 20 mM for D-amino acids as serine, alanine, and the acidic amino acids so serine, alanine, and the acidic D-amino acids are preferred substrates for the enzyme.