Question

Sample Extinction Coefficient L / (mole*cm) A260 A280 A320 A260/A280 OD260 MW Uknown 1 196800 .33...

Sample Extinction Coefficient L / (mole*cm) A260 A280 A320 A260/A280 OD260 MW

Uknown 1

 196800 .33 .21 .1 1.57 29 6167
Unknown 2 191800 .32 .21 .11 1.52 29 6311
Unknown 3 190200 .34 .21 .1 1.62 28 6127

Dilution factor of 1:30

(5 part sample / 150 part solution)

Given this set of data, how to calculate concentration of the unknowns?

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Answer #1

Solution:

By beer-lamberts law, A = Elc                        {E ------> extinction co-efficient}

c = A / El

Sample Extinction Coefficient L / (mole*cm) A260 A280 A320 A260/A280
unknown 1 196800 0.33 0.21 0.1 1.57
unknown 2 191800 0.32 0.21 0.11 1.52
unknown 3 190200 0.34 0.21 0.1 1.62
unknown 1 c = A / El 1.67683E-06 1.06707E-06 5.0813E-07 7.9776E-06
unknown 2 1.6684E-06 1.09489E-06 5.73514E-07 7.9249E-06
unknown 3 1.78759E-06 1.1041E-06 5.25762E-07 8.5174E-06
dilution 1/30 = 1:30 5.03049E-05 3.20122E-05 1.52439E-05 0.00023933
5.00521E-05 3.28467E-05 1.72054E-05 0.00023775
5.36278E-05 3.3123E-05 1.57729E-05 0.00025552
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