Question

A tissue culture plate experiment is done by adding 2 drops of hydroxylamine solution, 7 drops...

A tissue culture plate experiment is done by adding 2 drops of hydroxylamine solution, 7 drops of ligand solution, and 3 drops of iron solution to a well in the plate. If you want to repeat the experiment on a larger scale, what is the conversion factor from plate to cuvette assuming that you need to make 2.8 mL of total solution volume?

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Answer #1

Tissue culture well plates come in various sizes and shapes. The conversion factor can be calculated if we know the volume of well in the plate (working volume of the plate).

I have found out one from the internet and will show u the calculations according to it. If it doesnot match your requirement you can use the volume of well in your plate and calculate similarly.

Source: http://www.tpp.ch/page/produkte/09_zellkultur_testplatte.php

Considering F-base,

Working volume availaible = 0.34 ml

Conversion factor from plate to cuvette = Volume of solution to be made in cuvette / Volume of well in the plate = 2.8/0.34 = 8.24

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