Question

In chymotrypsin, a catalytic triad of asp, his, ser can be found in the active site....

In chymotrypsin, a catalytic triad of asp, his, ser can be found in the active site. Which of the amino acids could be substituted for another one with the least effect on the catalytic rate?

serine
histidine
none of them can be changed at all without losing all activity
histidine and aspartic acid could be changed with similar results
aspartic acid
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Answer #1

Mutagenesis studies on the related trypsin protein revealed that Ser195 and His57 contribute ∼1 million-fold toward the rate of catalysis for trypsin (Corey et al. 1992). The Asp102 contributes ∼10,000-fold as determined by mutagenesis experiments (Craik et al. 1987; Corey et al. 1992).

Please see journal "Unconventional serine proteases: Variations on the catalytic Ser/His/Asp triad configuration" , http://onlinelibrary.wiley.com/doi/10.1110/ps.035436.108/full

Hence aspartic acid amino acids could be substituted for another one with the least effect on the catalytic rate

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