Question

In a mixture of five proteins listed below, which should elute first in a size-exclusion chromatography under non-denaturing conditions?   Note that the size-exclusion resin can only resolve proteins from 25 kDa to 200,000 kDa​


A. RNA polymerase, MW 450,000 Da

B. Immunoglobin G, MW 145,000 Da
C. Serum albumin, MW 67,000 Da

D. Ribonuclease D, MW 17,000 Da

E. Cytochrome c, MW 13,000 Da

Answer 1