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A student begins to run a column at 4:00 pm. By 4:25 pm, the labs close,...

A student begins to run a column at 4:00 pm. By 4:25 pm, the labs close, and the student is not nearly finished with the separation. The student decides to cap up the column in order to finish it the next day. Why is this a bad idea?
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Answer #1

This technique of component separation is carried by continuous distribution of components between two phases i.e., one phase moves over the other in a continuous manner.

During this procedure the collecting vessels must be frequently switched and solvent levels need to be topped up.

it is extremely important to never let the column run dry at any time. this will allow air to enter the column, which will result in uneven bands and poor separation.

so if the student decides to finish the procedure the next day then the column wont be monitored in his/her absence and thus, the student wont be knowing the solvent level.

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