Question

In a protein, the spectrum in a 1 cm cuvette has an absorbance of 0.717 at 240 nm, and 0.239 at 280 nm. Assuming that these absorptions come from the aromatic Trp (ε240 = 1,960 M-1cm-1, ε280 = 5,380 M-1cm-1) and Tyr (ε240 = 1,500 M-1cm-1, ε280 = 11,300 M-1cm-1) residues, calculate the Tyrosine concentration. (Answer is 58 μM).

Answer 1