Question

Assume that a TLC analysis has shown that your target compound can be separated from a...

Assume that a TLC analysis has shown that your target compound can be separated from a compound mixture by a CC separation in toluene. Unfortunately, the compound mixture does not dissolve in toluene (i.e. you cannot apply the compound as a solution in toluene). After solubility tests you find that the compound mixture dissolves in ethyl acetate. Can you apply the compound mixture as a solution in ethyl acetate, followed by the elution in toluene? Please explain.

Homework Answers

Answer #1

The answer is No,

it would not be useful. Because Column chromatography depends on both Solubility and polarity.

Ethyl acetate is more polar than Toluene and also compound solubility is more in ethyl acetate.

once you apply compound mixture in ethyl acetate it will elute along with toluene because of solubility of the compound in ethyl acetate.

Hence you have to use the different percentage of non polar solvent (Alkanes preferably hexanes) mixture with ethyl acetate to purify through the column.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What was the reason for the addition of H2O (10 ml), followed by gentle heating? Draw...
What was the reason for the addition of H2O (10 ml), followed by gentle heating? Draw a mechanism equation for the reaction taking place. Why was the water added slowly and through the condenser? Here is my experiment... Experimental procedure Warning: acetic anhydride is corrosive and a lachrymator. When carrying it across the lab, when it is inside a flask, please temporarily stopper the flask. Reaction Place salicylic acid (200 mg = 0.200g) into a dry 25ml round bottom flask...
a)How is it possible to determine if CaCO3 is Cl- free after synthesis? b)How can the...
a)How is it possible to determine if CaCO3 is Cl- free after synthesis? b)How can the Cl- ions be remove from CaCO3 after synthesis? I should answer the questions from the following experiment but if you know the answer and you are sure, yo do not need to read experiment. Please answer correctly because i hav no chance to make wrong :(((( Physical and Chemical Properties of Pure Substances Objective The aim of today’s experiment is to learn handling chemicals...
Procedure Reaction 1: Dissolving the Copper 1. Obtain a clean, dry, glass centrifuge tube. 2. Place...
Procedure Reaction 1: Dissolving the Copper 1. Obtain a clean, dry, glass centrifuge tube. 2. Place a piece of copper wire in a weighing paper, determine the mass of the wire and place it in the centrifuge tube. The copper wire should weigh less than 0.0200 grams. 3. In a fume hood, add seven drops of concentrated nitric acid to the reaction tube so that the copper metal dissolves completely. Describe your observations in the lab report. (Caution, Concentrated nitric...
Please show me how to apply CVP analysis for this case study. FLY ASH BRICK PROJECT:...
Please show me how to apply CVP analysis for this case study. FLY ASH BRICK PROJECT: FEASIBILITY STUDY USING CVP ANALYSIS S. K. Mitra and Shubhra Hajela wrote this case solely to provide material for class discussion. The authors do not intend to illustrate either effective or ineffective handling of a managerial situation. The authors may have disguised certain names and other identifying information to protect confidentiality. This publication may not be transmitted, photocopied, digitized or otherwise reproduced in any...