Question

An enzyme-catalyzed reaction was carried out with the substrate concentration initially two hundred times greater than the Km for that substrate. After 5 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 30 micro mol. If, in a seperate experiment, TWO TIMES MORE enzyme and twice as much substrate had been combined, how long would it take for the same amount (30 micro mol) of product to be formed?

Answer 1

The enzyme converts 1% of substrate to product in 5 minutes

product concentration = 30 micromoles

the enzyme concentration is increased twice and substrate is also increased twice of initial amount.

The increase in concentration or amount of substrate will not affect the conversion rate. however the enzyme will do.

an enzym can convert substrate to 30micromoles in 5 minutes. The double amount of enzyme will require = 5/2 minutes

So the time will be =2.5 minutes