TLC Acquired Skills:
When to use
Predict elution order
Calculate Rf
How to improve the resolution of a TLC plate
(1) When to use
(A) In you are conducting a reaction how much reaction occure [happened] and how much starting material is consumed for this purpose we can use TLC.
Predict elution order :-
(1) In TLC Non Polar compounds run fast [Near o solvent front]
[Rf value more than 0.5]
(2) Polar spot won't be near to solvent front. [Rf value less than 0.5]
(3) Non polar spots elution first in TLC.
Non polar compounds Rf > 0.5
Polar compounds Rf < 0.5
Less polar travel faster.
How to improve the resolution of a TLC plate.
(a) Whatever may be we are putting spot on TLC we should take with dilution starting material.
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