An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km from these measurements. (Hint: Slope = rise/run and remember what Km represents!)
Vo (nM/S) | [S] (nM) |
30 | 100,000 |
29.5 | 10,000 |
29 | 1,000 |
28 | 200 |
25 | 150 |
20 | 100 |
10 | 50 |
5 | 20 |
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