Question

An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km...

An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km from these measurements. (Hint: Slope = rise/run and remember what Km represents!)

Vo (nM/S) [S] (nM)
30 100,000
29.5 10,000
29 1,000
28 200
25 150
20 100
10 50
5 20

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