Question

You will you use TLC to evaluate if your column chromatography separation was successful. The mobile phase is 10% acetone in heptane and the stationary phase of your TLC plate is silica. Which compound from your mix would you expect to see traveling further from the baseline of the plate? why?

Answer 1

Silica is exposed with polar functional groups on it's surface so any compound polar binds to it strongly and a non polar compound binds to it weakly.

When a mobile phase is run, leass polar compound will move further from the baseline and more polar compound sticks to the baseline or moves further from the baseline slower than the non polar compound because it has strong interaction with the polar functional groups of the silicca plate.

Since you have not mentioned any particular mixture of compounds I gave a general explanation. Based on this concept mentioned above i hope you can sort out which compound moves further and which do not, from a given mixture of compounds.