The detection limit for caffeine analysis must be calculated. Eight absorbancereadings from a blank solution are 0.1239, 0.1098, 0.1182, 0.1150, 0.1219, 0.1251, 0.1199,and 0.1099. The slope of a calibration curve is 0.59 /mM. What is the detection limit?
detection limit = 3X STANDRD DEVIATION / SLOPE OF THE CALIBRATION LINE
for calculating standard deviaton, first mean of sample need to be calculated which is (0.1239 +0.1098+ 0.1182 +0.1150 + 0.1219 + 0.1251 +0.1199 + 0.1099)/8 =0.117963
take differences of each sample from Arithmetic mean needs to be taken
the values are
they are (absorbance of particular point -average)
0.006038 |
-0.00806 |
0.000338 |
-0.00286 |
0.004038 |
0.007237 |
0.001238 |
-0.00796 |
Their squares
3.64514E-05 |
6.50039E-05 |
1.13906E-07 |
8.19391E-06 |
1.63014E-05 |
5.23814E-05 |
1.53141E-06 |
6.34014E-05 take the quare of averages give |
0.000243379
Standard deviation= sqrt* {0.000243379/(N-1)} where N= number of samples
Srandad deviation= 0.00589
deteaction limt= 3*0.00589/0.59=0.029949 mM
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