Question

What are the two main differences between Bradford protein assay and direct protein assay?

What are the two main differences between Bradford protein assay and direct protein assay?

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Bradford protein assay

It is based on the chemical reaction between Coomassie brilliant blue G-250 dye and proteins in solution

The dye binds to arginine, tryptophan, tyrosine, histidine, and phenylalanine residues and is highly sensitive

Direct protein assay

Direct protein assay needs no chemical reaction to be performed

This is based on the fact that the aromatic amino acids (tryptophan and tyrosine) show a peak in the UV-vis spectrum around 280 nm and has a moderate sensitivity only

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
The Bradford assay is especially sensitive to arginine residues.  If you have a protein with a high...
The Bradford assay is especially sensitive to arginine residues.  If you have a protein with a high percentage of arginine How will this affect your absorbance values? . B. What might you do to get more accurate protein concentration values?
Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most...
Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most accurate estimate of protein concentration. Explain why A280can be used to measure protein concentration. Is this method better suited to complex samples or purified proteins? After preparing a standard curve of diluted BSA protein in a BCA assay you get a trendline with the equation A562 = 0.0011[BSA] (mg/mL) + 0.0378. Rearrange the equation to solve for [BSA] (mg/mL). If a 1:8 dilution of...
For the Biuret, Lowry, Bradford, and BCA Assay: Why might you expect the different methods for...
For the Biuret, Lowry, Bradford, and BCA Assay: Why might you expect the different methods for determination of protein concentration to give different results?
Compare and contrast the Biuret reaction, BCA, and Bradford assay in chemical detail. Explain if there...
Compare and contrast the Biuret reaction, BCA, and Bradford assay in chemical detail. Explain if there are any pros or cons of each method, and why a protein scientist would prefer one method to another. Finally, suggest and describe other types of protein quantifications methods not mentioned here.
Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere...
Although the technical manual for the Bradford assay kit claims that very few lab reagents interfere with the assay, what control experiment can you do to determine whether a particular reagent interferes with the Bradford assay or not?
For the Bradford Assay, the instructor will make a Bradford reagent dye by mixing 50 mL...
For the Bradford Assay, the instructor will make a Bradford reagent dye by mixing 50 mL of 95% v/v ethanol with 100 mg of Coomasie Blue followed by the addition of 50mL of 85% v/v phosphoric acid. This entire mixture is then diluted to 1 liter with water. What is the final concentration of phosphoric acid?
What are two main differences between ADSL and SDSL?
What are two main differences between ADSL and SDSL?
1a.) Collagen is a major protein in connective tissue and the extracellular matrix. Both the Bradford...
1a.) Collagen is a major protein in connective tissue and the extracellular matrix. Both the Bradford assay and absorbance at 280 nm cannot be used to determine the concentration of collagen. Why is this? No more than two or three sentences, please 1b.) The old way of determining the amino acid composition of a protein involved hydrolyzing the peptide bonds joining the amino acids, separating the amino acids by chromatography, then determining the relative amounts of each. How would a...
You are performing a Bradford assay. You construct your standard curve (concentration in mg/mL on the...
You are performing a Bradford assay. You construct your standard curve (concentration in mg/mL on the x-axis, Absorbance on the y-axis) and derive a trendline with an equation of y = 0.25x (the intercept is set to zero because you blanked your spectrophotometer correctly). You dilute protein A using the volumes given below, to a final volume of 1000 μL, and measure the absorbance: 6 μL to 1000 μL = Abs 0.04 80 μL to 1000 μL = Abs 0.15...
In one or two paragraphs describe the main differences between the two systems between IFRS &...
In one or two paragraphs describe the main differences between the two systems between IFRS & GAAP
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT