Question

why must use semi log to graph protein migration in electrophoresis

Answer 1

The proteins will appear as blue bands. The size of the most abundant proteins can
be determined by setting up a standard curve using the size of the molecular weight
markers and the distance each marker band migrated from the well. Measure the distance
that each band of protien migrated from the bottom of the well to the middle
of each band . Measure the distance that the dye front migrated in each lane and record
CALCULATE THE RELATIVE MOBILITY (Rf) for each protein by dividing the distance migrated
by the distance the dye front migrated.the distaance each protien migrated the molecular
weight of each protien. Use semi log graph paper to graph the standard curve by placing the
molecular weight of each marker on the axis of the graph and Rf value on the X-AXIES