Question

considering a best fit line equation of y=1.0029x - 0.264, where concentration is in units of micrograms/milliliters, what concentration was present for a solution with an absorbance of 38?

Answer 1

considering a best fit line equation of y=1.0029x - 0.264, where concentration is in units of micrograms/milliliters, what concentration was present for a solution with an absorbance of 38?

If x represents concentration, y represents absorbance

So here y = 38, then x is solved as follows

38 = 1.0029x - 0.0264

Adding 0.0264 both sides we get

38.0264 = 1.0029x

Dividing by 1.0029 on both sides we get

x = 37.9

So concentartion of solution which absorbs 28 would be 37.9 ug/mL