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In order to make a Chromatin Immunoprecipitation (ChIP) dilution buffer, you need to make some concentrated...

In order to make a Chromatin Immunoprecipitation (ChIP) dilution buffer, you need to make some concentrated stock solutions first. Describe how to make the following solution: make 500 mL of a 10% SDS stock solution (SDS is a powder with a FW: 288.372 g/mol).

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Answer #1

mass of solute = volume of solution* W/V%

                    = 500*10/100 = 50gm

molarity   = weight of solute*1000/Gram molar mass*volume of solutionin ml

              = 50*1000/288.372*500

               = 0.3467M >>>>> answer

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