In Size exclusion chromatography, the components were separated on the basis of the size of particles present in the compounds. Different fractions collected from the compound will belong to a set of particular size component. Due to the difference in size of the fraction particles, they come out with a different rate as separated fractions. So these fractions will belong to a particular peak appear in the chromatograph. Sometimes, these fractions will come in the individual color of the component.
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