Question

The A260nm of a DNA solution is 0.12. How much of this DNA solution and a...

The A260nm of a DNA solution is 0.12. How much of this DNA solution and a 5.5X loading dye should you add to 15µL of water to obtain a sample which contains 30ng of DNA in 0.5X loading dye? (A260nm of 1.0 = 50 µg/mL DNA)

Answer is 5µL DNA and 2µL dye, please show work

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Homework Answers

Answer #1

Let

y ul of DNA and z ul of dye is added to 15 ul of water

so

final volume = 15 + y + z

now

consider the loading dye

we know that

during dilution

M1V1 = M2V2

so

5.5 x z = 0.5 x ( 15 + y + z)

5.5z = 7.5 + 0.5 y + 0.5z

5z = 7.5 + 0.5y


given

1.0 = 50 ug / ml DNA

so

0.12 = 6 ug / ml DNA = 6 ug / 1000 uL DNA

0.12 = 0.006 ug / uL DNA

now

given

amount of DNA = 30 ng = 0.03 ug

so

0.03 / y = 0.006

y = 5

now

5z = 7.5 + 0.5 y

so

5z = 7.5 + 2.5

z =2

so

5 ul of DNA and 2 ul of dye should be taken

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