Question

what kind of particular information can scientist able to obtain from the SDS gel. From the NATIVE gel. Compare and contrast

Answer 1

The molecular weight could be estimated with SDS PAGE. Since the mobility of a substance in a gel is influenced by both charge and size, in SDS PAGE samples are treated in order to have a uniform charge, so electrophoretic mobility in SDS PAGE depends mainly on size. SDS PAGE is carried out in the presence of an anionic detergent sodium dodecyl sulfate (SDS) and a reducing agent mercaptoethanol (BME). SDS disrupts the secondary, tertiary and quaternary structure of the protein to produce a linear polypeptide chain coated with negatively charged SDS molecules. BME contributes with protein denaturation by reducing all disulfide bonds. On the other hand in Native PAGE proteins are prepared in a non-reducing and non-denaturing sample buffer (neither SDS nor BME in the sample buffer and in the gel), which maintains both the proteins