Question

Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K_a = 5.01 × 10^?9 and pK_a = 8.3)

(a) Calculate the pH of the Tris buffer after mixing 13.0 mL of 0.15 M HCl solution with 25.0 mL of 0.10 M Tris.

(b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00017 mol of H⁺ was produced. What is the pH of the buffer at the end of the reaction?

(c) What would be the final pH if no buffer were present?

Answer 1