Question

You have 2L of 10X TBH stock buffer. How much water and 10X TBE stock should be mixed together to make 10L of 1X TBE buffer?

Answer 1

8L of water and 2L of

v1c1=v2c2 v1=?

c1=10X ,v2=10 L ,c2=1X ,(v1)(10X) = (10 L)(1X) v1 = (10 L)(1X) = (10 L)(1X) = 1 L V1 tells you how much stock (that’s the 10X (10X) (10X) TBE) to add. You still need to determine how much water to add.

the total volume of buffer you’re making (10 L). Just subtract! 10 L – 2 L = 8L So, mix together 2 L of the 10X TBE stock and 8 L of water to make 10 L of 1X TBE buffer.

. The rest (10 L – 2 L) should be water. So, mix together 1 L of the 10X TBE stock and 9 L of water to make 10 L of 1X TBE butter.