Question

Average human blood contains 300 nmoles of hemoglobin () per liter of plasma and 2.2 mmol per liter of whole blood. Calculate the molar concentration in each of these media.

Molar concentration in plasma = M and nM

Molar concentration in whole blood = M and mM

pHb in plasma and human serum.

pHb in plasma =

pHb in human serum =

Answer 1

In plasma:
n = 300 nmoles
V= 1 L
Molar concentration in plasma =300 nmoles/1L = 300 nM = 3*10^-7 M
pHb in plasma = -log [Hb]
              = -log (3*10^-7)
              = 6.52

In whole blood.:
n = 2.2 mmol
V= 1 L
Molar concentration in plasma =2.2 mmoles/1L = 2.2 mM = 2.2*10^-3 M
pHb in human serum = -log [Hb]
                   = -log (2.2*10^-3)
                   = 2.66