Question

Preparing Buffers

BUFFER	pKa1	pKa2	pKa3	Formula Weight
Acetate	4.76			136.1
CAPS	10.4			2221.3
Citrate	3.06	4.74	5.40	294.1
HEPES	7.55			238.3
Phosphate	2.12	7.21	12.32	142.0
Tricine	8.15			179.2
TRIS	8.3			121.1

I need help calculating how many grams do I need to weigh out to make 100mL of a 0.1M buffer for each of the following buffers.

Answer 1

Preparing Buffers

BUFFER	pKa1	pKa2	pKa3	Formula Weight
Acetate	4.76			136.1	136.1 grams in 1000ml gives one molar buffer concentration so to get 0.1 molar conc we have to add 1.36 grams in 100ml water to get 0.1 molar concentration M1v1 = M2V2 1molar = 136.1 in 1000ml 0.1 molar 13.6 1in 1000ml and 1.36 in 100ml
CAPS	10.4			2221.3	22.213 grams in 100ml gives 0.1molar concentration
Citrate	3.06	4.74	5.40	294.1	2.94 grams in100m
HEPES	7.55			238.3	2.383 grams in100m
Phosphate	2.12	7.21	12.32	142.0	1.42 grams in100m
Tricine	8.15			179.2	1.792 grams in100m
TRIS	8.3			121.1	1.211 grams in100m