Question

a. In gas chromatography which parameter is changed to alter retention factors of the solutes? b....

a. In gas chromatography which parameter is changed to alter retention factors of the solutes?
b. In liquid chromatography which parameter is changed to alter retention factors of the solutes?
c. Why does the use of an internal standard with chromatography give more accurate and precise results?

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Answer #1

Hello there.

Below is the answer to your question.

a. In gas chromatography, the solute retention factor can be changed by changing the temperature of the column, or by changing the length of the column or by changing the stationary phase nature that is used in the column.

b. In liquid chromatography, the solute retention factor can be altered by adjusting the solvent strength of the mobile phase, which includes modifying the amount of organic solvent in the mobile phase mixture.

c. The use of internal standard with chromatography give more accurate and precise results as volume errors will be difficult to predict and control. And, correct quantitative analysis will be obtained.

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