Question

I am currently working on a Breast Cancer Model research as my undergraduate research,

I will need to prepare a solution of TPA( 12-O-tetradecanoylphorbol-13-acetate) dissolved in Acetone to promote the tumor growth after the intiation stage. I am planning to make about 10 mls of the TPA solution to inject the rats twice a week for few weeks. If each rat should be treated with 10 μg of TPA in 200 μl of acetone which equals 0.2 mls, what are the quantities needed to prepare the solution if I want to make 10 mls of the solution with the above concentrations?

Answer 1

SOLUTION:

CONCEPT: Determine the concentration of solution which you are giving to rates in terms of μg per mL

Remember the conversion of units which is important in your case.

1000μL = 1mL

100μL = 0.1mL

200μL = 0.2mL

Each rat should be treated with ( Or You have dissolved) 10μg of TPA in 200μL or 0.2mL of acetone

It means 0.2mL of solution contain 10μg of TPA

one mL of solution will contain 10 / 0.2 = 50μg

Above result is important. It means 1mL of your solution contains 50μg of TPA ( This is actually concentration which you are giving to rates when you dissolve 10μg in 200μL of acetone)

Therefore if you want to give 10mL of solution to rates the amount of TPA = No. of mL X amount in one mL

= 10 X 50 = 500μg

Therefore dissolve 500μg in 10ml Or 10,000μL of acetone and adminster it to rates.