A specific serine residue on a protein is the site of phosphorylation of an ATP-dependent protein kinase. If the γ-oxygens of ATP were all 18O, how many labeled oxygens would the phosphorylated serine have (i) after it was phosphorylated and (ii) after a phosphatase catalyzed the hydrolysis of the phosphoryl group?
none of the other choices is correct |
1 and 4 |
3 and 0 |
0 and 3 |
0 and 4 |
4 and 0 |
1 and 3 |
4 and 1 |
3 and 1 |
Answer A specific serine residue on a protein is the site of phosphorylation of an ATP-dependent protein kinase. If the γ-oxygens of ATP were all 18O, how many labeled oxygens would the phosphorylated serine have (i) after it was phosphorylated is 4 and (ii) after a phosphatase catalyzed the hydrolysis of the phosphoryl group is 0 . As the mass spectrometers were operated in the data-dependent mode with dynamic exclusion enabled (repeat count, 1; exclusion duration, 0.5 min).
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