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This mystery reaction (Cysteine Hydrochloride with Acetone) is reversible. How might the equilibrium affect the outcome...

This mystery reaction (Cysteine Hydrochloride with Acetone) is reversible. How might the equilibrium affect the outcome of the ninhydrin and Eliman's tests?

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Answer #1

Ellman's test is used to quantify the number or concentration of thiol group in given compound. Thiol reacts with Ellman's reagent (5,5'-dithiobis-(2-nitrobenzoic acid)) and cleaves the disulfide bond to give 2-nitro-5-thiobenzoate which again ionizes to dianion (yellow color) in water at different pH(neutral and alkaline).

Ninhydrin used to detect ammonia or primary and secondary amines. Reacting with these free amine group ninhydrin gives a deep blue or purple color known as "Ruhemann's purple" stain.

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