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Should acetonitrile and water be pre-mixed, or can they be added sequentially? How will you filter...

Should acetonitrile and water be pre-mixed, or can they be added sequentially? How will you filter your reaction?

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Answer #1

Actonitrile forms an azeotrope with water.Acetonitrile and water are completely miscible.
Maintain the pH of the aqueous phase, then mixed in the organic phase. The best way to maintain desired pH is by adding acid.
If mobile phase is pre-mixed over time due to selective evaporation water:organic ratio will change, causing retention in time variability.

Membrane-filteris used in any mobile phases that contains a dissolved solid, even though the solvents are pre-filtered by HPLC (High performance liquid chromatography).

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