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4. You are provided with a 10 μg sample of gel-purified DNA. (The sample contains only...

4. You are provided with a 10 μg sample of gel-purified DNA. (The sample contains only one DNA sequence.)

(a)Describe how electrophoresis was used to prepare this sample and specify on factors that affect electrophoretic mobility. How can the size of the DNA molecule be estimated using this technique?

(b)How would you go about determining if the DNA molecule was single-stranded or double-stranded? Be specific with regards to your proposed experiment(s) and the properties/data you are monitoring. Provide example graphs or images in support of your approach.

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