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please answer the following questions thank you. 1. Why does PVA have to be activated by...

please answer the following questions thank you.

1. Why does PVA have to be activated by FMP? 



2. Why does it take longer to reach a steady potential with higher urea concentrations?

3. Why is the calibration curve dependent on ionic strength? 


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Answer #1

1.PVA have to be activated by FMP in order to make it useful for various purposes.It has to be activated by FMP in order for its smooth functioning.

2.It takes longer to reach a steady potential with higher urea concentrations because urea concentration is increasing enormously .Protein hydrolysis yields urea thus its concentration goes on increasing.

3.Calibration curve depends on ionic strength because at high ionic strength fluorescence intensity is high which helps it to predict the concentration of analyte in a sample.

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