Question

(a) How many theoretical plates produce a chroma- tography peak eluting at 12.83 min with a...

(a) How many theoretical plates produce a chroma- tography peak eluting at 12.83 min with a width at half-height of 8.7 s?

(b) The length of the column is 15.8 cm. Find the plate height.

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Answer #1

a)

number of plates = 16 ( peak elutiing / half height )^2

                            = 16 (12.83 / 8.7)^2

                           = 34.8

b) plate height = length / number of plates

                           = 15.8 / 34.8

                          = 0.454 cm

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