Question

A pharmaceutical company is interested in the development of a new analytical strategy for the analysis of drug samples containing a wide range of hydrophobic and hydrophilic drug molecules. They either have boiling points of over 1100 ^oC and some decompose at temperatures slightly lower than this. Like many drugs, they fluoresce.

a. What chromatographic technique would you use for this separation?

b. What is the basis for separation in the technique you selected?

c. Name and briefly discuss the operating principle of a suitable detector you would use for this separation.

d. Why did you select this particular detector?

e. If the detector you selected in part b was unavailable, what other could you use? Why?

f. What mobile phase or combination of mobile phases would you select?

Answer 1