Question

Which two chemical components in a protein sequence largely determines a protein's absorption of light at 280 nm?

Answer 1

Tyrosine , Tryptophan, Cysteine are the chemical components in a protein sequence that are the reason for the absorption of light at 280 nm with their molar coefficients dreassing in the same order.

The molar absorption coefficient of the proteins itself at 280 nm will depend on the relative concentrations of each of these three amino acids.

So different proteins have different absorption coefficients and even the wavelength of the absorption differ for each

This helps in identifying the different types of proteins.

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