Question

Which of the following statements about DNA microarrays is not true?

(a) Using automated processes, DNA chips can be made that contain thousands of different DNA segments.

(b) Photolithography is a method to make DNA microarrays that utilizes light to remove protecting groups during nucleic acid synthesis.

(c) DNA strands on the microarrays will bind with identical strands of fluorescently labeled DNA segments, indicating the sequence of said strand.

(d) DNA chips utilize fluorescence to identify DNA-DNA or RNA-DNA interactions.

(e) Hybridization is the fundamental concept behind the use of DNA microarrays.

Answer 1

DNA microarray generally do the following functions:

- Gene chip are used to monitor thousands of genes simultaneously

- Hybridize tool to study which genes are transcribed into mRNA (which genes are on)

- Microarrays are commonly used to study gene expression patterns.

Therefore , (a),(b),(d) and (e) are true but

the statement (c) is not true

DNA strands on the microarrays will bind with identical strands of fluorescently labeled DNA segments, indicating the sequence of said strand. As DNA microarrays does not carry a detectable label.