Question

Can someone please find me a FREE peer reviewed article that talks about the the implications/ factors effecting gel-filtration column chromotography. Also, if you can provide your knowldege of implications/ factors effecting gel-filtration.

Thank you!

Answer 1

, increasing the column length gives better resolution, and increasing the column diameter increases column capacit

. Proper column packing is important for maximum resolution: An over-packed column can collapse the pores in the beads, resulting in a loss of resolution. An under-packed column can reduce the relative surface area of the stationary phase accessible to smaller species, resulting in those species spending less time trapped in pores. Unlike affinity chromatography techniques, a solvent head at the top of the column can drastically diminish resolution as the sample diffuses prior to loading, broadening the downstream elution.