Question

when using a quality control sample in HPLC analysing, what is the acceptable percentage error that can be get from it ? please give one reference to support your answer

Answer 1

to find the acceptable percentage error we first need to analyse the detection limit of HPLC. the limit up to which the sensitivity of the instrument to detect the sample of our choice. we can use the signal to noise ratio from the samples with known concentrations of analyte starting from the lowest and increasing to a certain value. Now we can run the samples in HPLC to see what is the lowest concentration that gets detected and take their ratio. The acceptable ratio is between 2-3.

also from the graph plotted for various concentration, we can use this formula:

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