Question

Predict the modes of LDH A inhibition by oxalic acid, oxamate, and ethyl oxamate. Justify your answer.

Answer 1

Modes of inhibition of LDH (lactate dehydrogenase) by oxalic acid is a competitive inhibition. Oxamate is a noncompetitive inhibitor. ethyloxamate shows uncompetitive type of inhibition. The type of inhibition shown in the cases of oxalix acid, oxamate and ethyl oxamate are confirmed by plotting inverse of reaction velocity of y-axis versus inverse of LDH concentration on x-axis. calculating the Km and Vmax from each plot. In case of oxalic acid, th Vmax remains the same whereas, the Km increases. In case of oxamate the Km remained the same whereas, the Vmax was seen to decrease, and finally with ethyl oxamate the both the Km and the Vmax for the reaction with the inhibitor added was seen to be lower than the without the inhibitor. Thus confirming the types of inhibition from the experimental results.