Question

Calculate the I.U./L of alkaline phosphatase in each specimen

I.U./L= (Delta A/Delta t) X (10^6/b.E) X (T.V./S.V)

b=light patch in cm

E=molar absorptitivty (L/(mole)(cm))

T.V.= total reaction volume

S.V.= specimen volum

Use the value for molar absorptivity of p-nitrophenol that the instructor has determined to be the appropriate value for the class to use. *.0001

Control 2 Absorbance @ 405 nm 0.044

Total Volume 5.535 ml

Specimen Volume 2 ml

Minutes 20

1 cm cuvet

Answer 1