Which method of ionization would you suggest for each of the following analytes? Explain your reasoning.
a) Dioxin
b) Horseradish peroxidase
c) Steel shavings
a) Dioxins are volatile and hence Electron Impact ionization
(EI) method or Chemical Impact (CI)
can be used to analyze the analyte where fragmentation
is more.
b) Horseradish peroxidase is an enzyme present in horseradish which
is used to make wasabi.
Electronspray Ionization (ESI) is used where no fragmentation takes
place.
c) Steel shavings contain metals like iron, nickel, manganese etc
in different proportions.
Many elements analysis can be done by using Inductively coupled
plasma (ICP) method.
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