Question

Penny sample absorbance value has to be the highest and lowest absorbance values for standard solutions. Explain why this is important.

Answer 1

Absorbance more than one is possible.

A=log10 Io/I

Low absorbance :

Absorbance zero means no light is absorbed (usually blank)  

here the intensity of incident light is equal to the intensity of transmitted light hence the ratio is one and

log 1 = zero absorbance.

High absorbance:

Absorbance one means the 90% light has been absorbed. hence the ratio is 100/10 and log 10 is one.

at times absorbance could be more than 90% in those cases the absorbance would be more than 1.

significance :low absorbance shows that no light is absorbed and high absorbance shows that light has been absorbed more than 90%.the intensity of incident light is to intensity of reflected light