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Explain the principle that causes polyhistidine-tagged proteins to bind to resins such as Ni-NTA, and the...

Explain the principle that causes polyhistidine-tagged proteins to bind to resins such as Ni-NTA, and the reason that imidazole can elute tagged proteins.

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Answer #1

The polyhistidine tagged proteins bind to resins such as Ni-NTA (NTA=nitrotriacetate) which is the most commonly used ligand for IMAC,  because this particular metal and chelation has better binding properties and suitability for specific applications of IMAC.

Histidine-tag purification uses the purification technique of immobilized metal affinity chromatography IMAC.Imidazole competes with the his-tag for binding to the metal-charged resin and thus is used for elution of the protein from an IMAC column.

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