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sketch a block diagram of a flurescence spectrophotometer and briefly state why selectivity is better in...

sketch a block diagram of a flurescence spectrophotometer and briefly state why selectivity is better in this type of analysis than that of conventional spectrophotometric determination?

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Answer #1

A true fluorescence spectrophotometer is an instrument in which two monochromators are used, one to supply the excitation radiation and the other to analyze the fluorescence emission. With dual monochromators the wave length of the excitation as well as the fluorescence can be determined and utilized selectively.

With such an apparatus, one can (a) choose any frequency of exciting light and measure the spectrum of the fluorescence emitted by the sample or (b) set the fluorescence monochromator on the wave length of the fluorescence band of the substance and observe how the intensity of this fluorescece varies with the frequency of the exciting light used.

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