Question

In the small popypeptide chain shown below Ala- Ser-CYS1- Lys- Cys2- Asn- Ala- Pro- Cys3- Ala-Phe-Cys4-Glu-His...

In the small popypeptide chain shown below

Ala- Ser-CYS1- Lys- Cys2- Asn- Ala- Pro- Cys3- Ala-Phe-Cys4-Glu-His

there is only one disulfide bond present. After treatment with trypsin, two polypeptide fragments were produced. After treatment with chymotrypsin, only one polypeptide was produced. in both treatments the disulfide bond remained intact.

Where is the disulfide bond located?

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The disulfide bond located between CYS2-CYS4 or CYS3-CYS4.

The trypsin digestion site is C-terminal side of the positively charged amino acids, such as Lys and Arg, but no Pro after Lys/Arg.

The chymotrypsin digestion site is C-terminal side of the bulky hydrophobic amino acids, such as Phe, Trp, Tyr, but not Pro after Phe/Trp/Tyr.

Since there are 2 fragments are formed on trypsin digestion, no disulfide bond is possible between CYS1-CYS2, CYS1-CYS3, CYS1-CYS4

Since there is only a single fragment on chymotrypsin digestion, there should be disulfide bond between CYS2-CYS4 or CYS3-CYS4. No disulfide bond between CYS2-CYS3.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-ser-gly-thr-stop start-val-ala-thr-thr-leu-tyr-cys-gly-arg-stop start-lys-asn-gly-phe-his-thr-arg-pro-gln-stop start-met-thr-asn-lys-pro-gln-ser-leu-arg-stop
Can you calculate the pI for the following peptide? Phe-Lys-Glu-Asp-Lys-Ser-Ala (note: there is one alpha carboxyl...
Can you calculate the pI for the following peptide? Phe-Lys-Glu-Asp-Lys-Ser-Ala (note: there is one alpha carboxyl at the C-terminus and one alpha-amino group at the N- terminus and don't forget to take into account the ionizable side chain groups!)
What would be the charge of this peptide, Asn-Arg-Met-Lys-His-Pro-Trp-His-Thr-Tyr-Ser-Gly-Glu-Leu-Val-Trp, at a pH of 1 and a...
What would be the charge of this peptide, Asn-Arg-Met-Lys-His-Pro-Trp-His-Thr-Tyr-Ser-Gly-Glu-Leu-Val-Trp, at a pH of 1 and a pH of 11?
The following decapeptide composition resulted from an amino acid analysis: Asn, Glu, Trp, 2 Thr, Met,...
The following decapeptide composition resulted from an amino acid analysis: Asn, Glu, Trp, 2 Thr, Met, Leu, Ala, His, Phe 1. Pepsin (cleaves on N-side of aromatics) yielded a tripeptide composition (Trp, Leu, Glu), a hexapeptide, and a free Thr. 2. Chymotrypsin (cleaves C-side of aromatics) treatment gave dipeptide composition (Thr, Trp), tripeptide composition (Glu, Phe, Leu), and a pentapeptide. The first amino of the pentapeptide has a secondary alcohol. 3. Elastase (cleaves C-side of Gly and Ala) resulted in...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT