Question

Biochem: The Sanger method for DNA sequencing uses a small amount of ddNTP in the presence...

Biochem:

The Sanger method for DNA sequencing uses a small amount of ddNTP in the presence of standard dNTPs. Why is only a small amount of each ddNTP used rather than 100% ddNTP? ​

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Sanger sequencing requires a single-stranded DNA template, a DNA polymerase, a DNA primer, normal deoxynucleosidetriphosphates (dNTPs), and modified nucleotides (ddNTPs) that terminate DNA strand elongation. These ddNTPs lack a 3′-OH group that is required for the formation of a phosphodiester bond between two nucleotides, causing the extension of the DNA strand to stop when a ddNTP is added. The DNA sample is divided into four separate sequencing reactions, containing all four of the standard dNTPs (dATP, dGTP, dCTP, and dTTP), the DNA polymerase, and only one of the four ddNTPs (ddATP, ddGTP, ddCTP, or ddTTP) for each reaction. After rounds of template DNA extension, the DNA fragments that are formed are denatured and separated by size using gel electrophoresis with each of the four reactions in one of four separated lanes.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1. How is complimentary DNA (cDNA) made from total RNA extract? What type of enzyme and...
1. How is complimentary DNA (cDNA) made from total RNA extract? What type of enzyme and primer is needed? What are the advantages of cloning a gene from its cDNA as opposed to the genomic DNA sequence (introns)? 2. If a PCR reaction replicates both strands of the DNA each cycle, this produces exponential amplification of the product. If only one strand is replicated (as in a Sanger sequencing PCR reaction) there is a linear growth in the product. Why...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot. Select one: a. Northern b. Western c. Northeastern d. Southern e. Southwestern 2. DNA sequencing by controlled termination of replication is called the _____ method. Select one: a. Sanger b. E. coli c. Restriction enzymes d. DNA Microarray e. Ligase f. Polymerase Chain Reaction g. Footprint h. Reverse Trancriptase i. Vector j. Expression k. Fluorescent l. cDNA 3. How...
12. If the DNA repair mechanisms fail to correct a defect in nucleotide sequencing, a permanent...
12. If the DNA repair mechanisms fail to correct a defect in nucleotide sequencing, a permanent change known as a __________________ may result. 13. As the enzyme helicase opens and “unzips” the two strands of DNA by breaking the hydrogen bonds, a Y-shaped ________________________ forms. a. Lagging strand b. Leading strand c. Okazaki fragment d. Replication fork 14. One large difference between transcription and translation between prokaryotes and eukaryotes is that, in prokaryotes: a. Translation occurs simultaneously with transcription b....
Bryson Company has accounts receivable of $125,000 at October 31, 2020, and uses the allowance method...
Bryson Company has accounts receivable of $125,000 at October 31, 2020, and uses the allowance method to account for uncollectible receivables. Based on experience, it estimates that 8% of its receivables will be uncollectible. (a) What journal entry would Bryson make to record the estimated uncollectible amount assuming the company already has an existing $1,500 debit balance in its Allowance account? (b) What journal entry would Bryson make to record the write-off of a $750 receivable? (c) What journal entries...
Amortize Discount by Interest Method On the first day of its fiscal year, Ebert Company issued...
Amortize Discount by Interest Method On the first day of its fiscal year, Ebert Company issued $19,000,000 of 5-year, 8% bonds to finance its operations. Interest is payable semiannually. The bonds were issued at a market (effective) interest rate of 10%, resulting in Ebert receiving cash of $17,532,812. The company uses the interest method. a. Journalize the entries to record the following: 1. Sale of the bonds. Round amounts to the nearest dollar. If an amount box does not require...
Marvel Parts, Inc., makes seat covers for small cars. The company uses a standard cost system...
Marvel Parts, Inc., makes seat covers for small cars. The company uses a standard cost system to help control its costs.   According to the standards that have been set for the seat covers, the factory should work 2,400 direct labor hours each month to produce 1,600 seat covers. The standard costs associated with this level of production are : Total Per Set of Covers Direct materials $42,080 $26.30 Direct labor $43,200 $ 27.00 During April, the factory worked 3,050 direct...
16. You digested a linear DNA fragment with a RE and the fragment has three recognition...
16. You digested a linear DNA fragment with a RE and the fragment has three recognition sites for the RE. How many fragments of DNA will be produced? A. three                B. four C. five D. six                     17. The RE HhaI cuts the recognition site 5’GCGC3’. The probability of having a cutting site for this enzyme is about one in every __________bp of genomic DNA. A. 100 B. 250                C....
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping...
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping interval. Based on a certain article, the mean of the x distribution is about $29 and the estimated standard deviation is about $7. (a) Consider a random sample of n = 40 customers, each of whom has 10 minutes of unplanned shopping time in a supermarket. From the central limit theorem, what can you say about the probability distribution of x, the average amount...
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping...
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping interval. Based on a certain article, the mean of the x distribution is about $12 and the estimated standard deviation is about $7. (a) Consider a random sample of n = 60 customers, each of whom has 10 minutes of unplanned shopping time in a supermarket. From the central limit theorem, what can you say about the probability distribution of x, the average amount...
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping...
Let x represent the dollar amount spent on supermarket impulse buying in a 10-minute (unplanned) shopping interval. Based on a certain article, the mean of the x distribution is about $41 and the estimated standard deviation is about $8. (a) Consider a random sample of n = 50 customers, each of whom has 10 minutes of unplanned shopping time in a supermarket. From the central limit theorem, what can you say about the probability distribution of x, the average amount...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT