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would a protein with a large extinction coefficient have a large fluoroescent signal? why?

would a protein with a large extinction coefficient have a large fluoroescent signal? why?

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Flourescence output is proportional to the product of the extinction coefficient at the particular wavelength and the flourescence quantum yield. If the extinction coefficient of the protein is large, the corrosponding flourescent signal will also be large. Ectinction coefficient is also related to absorbance by the formula: A= ecl, where A is absorbance, e is extinction coefficient, c is concentration and l is the length of the tube. Since absorbance spectrum is eqivalent to the flourescence exitation spectrum, it is proportionally related to e.

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