Question

Analytical Chem:

What component of the typical HPLC system guarantees that this is a highly precise technique when used properly?

a. Column, Injector loop ,UVVis detector, or Pump

b. Briefly explain why :

Answer 1

actually the important part of HPLC in solvent delivery sstem is pump, because its performance directly effects the retention time , reproducibility, and detector sensitivity.

the mobile phase is pumped under pressure from one or several reservoirs and flows hrough the column at a constant rate.

Inorder to achieve the effiecient separation of sample constituents, the column should be packed in such a way that highest number of theoretical plates are possible.

number of theoretical plates decides the efficency of column.