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Why are EI and CI techniques used in GC/MS and APCI or ESI techniques used in...

Why are EI and CI techniques used in GC/MS and APCI or ESI techniques used in LC/MS? What is the difference and why are they not interchangeable?

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Answer #1

By oleoresin extracted of using hexane in LC/MS for conforming lutein esters.

Running a parallel sample is saponified(i.e, with esters removed) which is intended to contain pure lutein.

Difference between ESI and APCI is,ESI is a source of ionization process that occur in mobile phase with addition of acid or basic compounds.

APCI is a source of ionization process that occur in a corona needle,this is performed for a electrical discharge in needle.

APCI is used to ionozation non polar molecules.

ESI is used in polar molecules or polar compounds.

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